Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias

Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias

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OBJECTIVES: The aim of this study Bike Parts - Cables was to analyze the immunolabeling of two cell cycle protein regulators, p53 and p21WAF1, in non-dysplastic leukoplakias with different epithelial alterations: acanthosis, hyperkeratosis and acanthosis combined with hyperkeratosis, and compare them with dysplastic leukoplakias.MATERIAL AND METHODS: This was a prospective cohort study involving 36 patients with oral homogeneous leukoplakias.excisional biopsies were performed and the patients remain under clinical follow-up.

The leukoplakias were divided into four groups: 6 acanthosis, 9 hyperkeratosis, 10 acanthosis combined with hyperkeratosis, and 11 epithelial dysplasias.Paraffin-embebeded sections were immunostained for p53 and p21WAF1.Five hundred cells from the basal layer and 500 from the parabasal layer were counted to determine the percentage of positive cells.

A qualitative analysis was also carried out to determine the presence or absence of immunohistochemical staining in the intermediate and superficial layers.Groups were compared with ANOVA (p0.05).

CONCLUSIONS: Our findings failed to differentiate the non-dysplastic lesions by means of p53 and p21WAF1 immunostaining, notwithstanding similar profiles between non-dysplastic and dysplastic Horse Wear » Halters » Bronc Halters leukoplakias were observed.